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Iindaba

Gubungela iingcebiso zesilayidi zeglasi

Izilayidi zinokwahlulwa ngokweendidi ezimbini: izilayidi eziqhelekileyo kunye ne-anti-detachment slides:
✓ Izilayidi eziqhelekileyo zingasetyenziselwa ukucoca i-HE, amalungiselelo e-cytopathology, njl.
✓ Izilayidi ezichasene ne-detachment zisetyenziselwa imifuniselo efana ne-immunohistochemistry okanye kwi-situ hybridization.
Umehluko omkhulu phakathi kwezi zibini kukuba kukho into ekhethekileyo ebusweni be-slide echasene ne-detachment eyenza izicubu kunye nesilayidi zibambelele ngokuqinileyo.
Ubungakanani bezilayidi zeglasi ezisetyenziswa ngokuqhelekileyo kwiimicroscopes yi-76 mm × 26 mm × 1 mm. Ukuba ubuso be-slide yeglasi ethengiweyo inee-arcs okanye i-protrusions encinci, ama-bubbles amakhulu omoya ahlala ebonakala kwicandelo emva kokutywinwa, kwaye ukuba ukucoceka kwendawo akwanele, kuya kubangela iingxaki. I-tissue iyachithwa, okanye umphumo wokuqwalasela awufanelekanga.
Izigqubuthelo zicekethekile, amacwecwe eglasi asicaba, aqhelekile ukuba sisikwere, arawndi, kunye noxande, athi abekwe phezu kwesampulu ejongwe phantsi kwemakroskopu. Ubunzima beglasi yokugubungela budlala indima ebaluleke kakhulu kwimpembelelo yokucinga. Andazi ukuba uzibonile iilensi zeZeiss. Ilensi nganye yenjongo ineeparamitha ezininzi ezibalulekileyo, kubandakanywa iimfuno zobunzima beglasi yokugubungela. .
1. I-0.17 kumzobo ibonisa ukuba xa usebenzisa le lens yenjongo, ubukhulu beglasi yokugubungela kufuneka ibe yi-0.17mm.
2. Ummeli onophawu oluthi “0″ akafuni glasi yokugquma
3. Ukuba kukho uphawu "-", kuthetha ukuba akukho glasi yokugubungela.
Kuqwalaselo lweconfocal okanye uqwalaselo lolwandiso oluphezulu, eyona ixhaphakileyo ngu “0.17″, nto leyo ethetha ukuba kufuneka sithathele ingqalelo ubukhulu be-coverlip xa sithenga ii-coverlips. Kukho neenjongo ezinezindandatho zokulungisa ezinokuthi zitshintshwe ngokobunzima be-coverlip.
Iindidi eziqhelekileyo ze-coverlips kwimarike zezi:
✓ #1: 0.13 - 0.15mm
✓ #1.5: 0.16 - 0.19mm
✓ #1.5H: 0.17 ± 0.005mm


Ixesha lokuposa: Sep-23-2022