Iindaba

1 Indlela ye-smear yindlela yokwenza ifilimu enxibe ngokulinganayo imathiriyeli kwi-aisilayidi seglasi. Izixhobo ze-smear ziquka izinto eziphilayo ezineseli enye, i-algae encinci, igazi, i-bacterial Culture fluid, izicubu ezikhululekile zezilwanyana kunye nezityalo, i-testis, i-anthers, njl.
Nika ingqalelo xa uthambisa:
(1) Isilayidi seglasi kufuneka sibeicocekile.
(2) Isilayidi seglasi kufuneka sibe mcaba.
(3) Ukwaleka kufuneka kube yinto efanayo. I-smear liquid iwiswa ngasekunene kumbindi wesilayidi, kwaye isasazwe ngokulinganayo nge-scalpel blade okanye i-toothpick.
(4) Ukwaleka kufuneka kube kubhityile. Sebenzisa esinye isilayidi njengesityhala, uze utyhale ngobunono ukusuka ekunene ukuya ekhohlo ecaleni komphezulu wesilayidi apho isisombululo se-smear sithontsizwa khona (i-engile phakathi kwezilayidi ezibini kufuneka ibe yi-30 ° -45 °), kwaye usebenzise umaleko obhityileyo ngokulinganayo.
(5) Zilungisiwe. Ukulungiswa, ukulungiswa kweekhemikhali okanye indlela eyomileyo (ibhaktheriya) ingasetyenziselwa ukulungiswa.
(6) Ukudaya. I-Methylene blue isetyenziselwa iibhaktheriya, ibala likaWright lisetyenziselwa igazi, kwaye ngamanye amaxesha iodine ingasetyenziswa. Isisombululo sokudaya kufuneka sigubungele yonke indawo epeyintiweyo.
(7) Hlamba. Gcoba ngephepha elifunxayo okanye ithowusti yome.
(8) Vala ifilimu. Ukugcina ixesha elide, tywina izilayidi ngeCanadian gum.
2. Indlela yethebhulethi yindlela yokwenza amaphepha ngokubeka izinto zebhayoloji phakathi kwesilayidi seglasi kunye ne-cover slip kunye nokufaka uxinzelelo oluthile lokusabalalisa iiseli zezicubu.
3. Indlela yokuxhoma yindlela apho imathiriyeli yebhayoloji itywinwa iyonke ukwenza imizekelo yesilayidi. Le ndlela ingasetyenziselwa ukwenza ii-mounts zexeshana okanye ezisisigxina. Izixhobo zokulayisha izilayi ziquka: izinto ezincinci ezifana neChlamydomonas, Spirogyra, Amoeba, kunye nematodes; I-Hydra, i-epidermis yamagqabi yezityalo; amaphiko, iinyawo, umlomo wezinambuzane, iiseli ze-epithelial zomlomo womntu, njl.
Ingqalelo kufuneka ihlawulwe kulungiselelo lwendlela yesilayidi:
(1) Xa ubambe isilayidi, sifanele sibe sicaba okanye sibekwe eqongeni. Xa ukuvuza amanzi, ubungakanani bamanzi kufuneka bufaneleke, ukwenzela ukuba uhlanganiswe nje ngeglasi yokugubungela.
(2) Izinto eziphathekayo kufuneka zityhilwe ngenaliti yokuqhawula okanye i-tweezers ngaphandle kokugqithisa, kwaye ifakwe kwindiza enye.
(3) Xa ubeka iglasi yokugquma, vala kancinane ithontsi lamanzi ukusuka kwelinye icala ukuthintela amaqamza omoya ukuba angabonakali.
(4) Xa uhlambalaza, beka ithontsi yesisombululo se-staining kwelinye icala leiglasi yokugubungela, kwaye uyifunxe kwelinye icala ngephepha elifunxayo ukwenza umfuziselo ophantsi kweglasi yokugquma ube nombala olinganayo. Emva kokufakwa kwemibala, sebenzisa indlela efanayo, uwise ithontsi lamanzi, ufunxe isisombululo se-staining, kwaye ujonge phantsi kwe-microscope.