Isitya se-petri sisitya selabhoratri esiqhelekileyo, esine-flat disc-shaped bottom kunye nesigqubuthelo, ngokukodwa esenziwe ngeplastiki kunye neglasi, kunye neglasi ingasetyenziselwa izinto zezityalo, inkcubeko ye-microbial kunye nenkcubeko yokubambelela kwiseli yezilwanyana.Uninzi lweplastiki iyalahlwa, ifanelekile kwi-inoculation yebhubhoratri, i-streaking, kunye nokuhlukaniswa kweebhaktheriya zokulima izinto zezityalo.
Indlela/inyathelo:
1
Izitya zePetri zidla ngokwenziwa ngendlela eqinileyo yenkcubeko yepleyiti (leyo yimvelaphi yegama lepleyiti yepleyiti).Imveliso yeplate medium kukunyibilikisa efakwe inzalo medium agar ngamanzi ashushu (inyumba), susa uvavanyo ityhubhu umqhaphu plug, Dlulisa umlomo ityhubhu phezu kwedangatye isibane utywala, uze uvule kancinane isiciko somqhaphu inzalo. isitya inkcubeko, ukuze umlomo ityhubhu yovavanyo ungangena nzulu.Isasazwa ngokulinganayo phantsi kwesitya kwaye igxininiswe ukufumana i-plate culture medium.
2
Ngenxa yokuba ukuveliswa, ukuphuhliswa kunye nokukhula kweebhaktheriya kuhambelana ngokuthe ngqo kwi-medium enikeziweyo (isondlo), ngokukodwa ukuhlolwa kobungakanani kunye nokuhlalutya, kunokubaluleka okuqinisekileyo kwinani lezondlo ezinikeziweyo.
3
Isixa sesondlo esibonelelwayo ngexesha lenkcubeko yebhaktiriya, nokuba iyafana, kwaye nokuba umphantsi wesitya se-petri usicaba kubaluleke kakhulu.Ukuba i-bottom ye-petri dish ayilingani, ukuhanjiswa kwe-agar medium kuya kuhluka ngokuxhomekeka ekubeni i-bottom ye-petri dish i-flat okanye ayikho.Unikezelo alunelanga, oluhambelana ngokusondeleyo nohlalutyo lobungakanani, ngoko ke i-bottom of the quantitative petri dish iyadingeka ukuba ibe flat ngokukodwa ngenxa yesizathu.Nangona kunjalo, ukubonakaliswa ngokubanzi (ukuhlolwa kweebhaktheriya, ukukhula kwekholoni, ukuzala, njl.), izitya eziqhelekileyo ze-petri zingasetyenziswa.
Ukulumkela
Emva kokucoca kunye ne-disinfection ngaphambi kokusetyenziswa, nokuba i-petri dish ihlambulukile okanye ayinayo impembelelo enkulu kumsebenzi, onokuchaphazela i-pH ye-medium.Ukuba kukho imichiza ethile, iya kuthintela ukukhula kweebhaktheriya.
Ixesha lokuposa: Nov-22-2022