Iindaba

Iipipettes ziqhele ukusetyenziswa kwiilabhoratri ukuhambisa umthamo wemililitha yolwelo, ukusuka kubuncinci be-1 ml ukuya kubuninzi be-50 ml. Iistraws zinokulahlwa kwiplastiki engcolileyo okanye ziphinde zisetyenziswe kwiglasi ekwazi ukuvuleka. Zombini iipayipi zisebenzisa i-pipette ukuze zifune kwaye zikhuphe ulwelo. Ubungakanani obuhlukeneyo beepipettes busetyenziswa kwiimvavanyo ezahlukeneyo kunye nepipette efanayo. Ngokomzekelo, iipipettes zibalulekile ekuxubeni izisombululo zeekhemikhali okanye ukumiswa kweeseli, ukuhambisa i-liquids phakathi kwezikhongozeli ezahlukeneyo, okanye i-plating reagents kwi-densities eyahlukeneyo. Ngethuba nje ingqalelo enonophelo ihlawulwe kumthamo we-liquid aspirated and expeed, i-pipettes ingaba sisixhobo esiluncedo kwibhubhoratri yokudlulisa ngokuchanekileyo imiqulu ye-milliliter yamanzi.

Iintlobo zeepipettes kunye namacandelo asisiseko seepipettes

Iipipettes idla ngokuba yityhubhu yeplastiki engasebenziyo enye; zisenokuthi zikwazi ukuzenzela, iityhubhu zeglasi ezisebenzisekayo.

Zonke iipayipi zisebenzisa i-pipette xa ibhobho.

I-pipette iphelisa isidingo sokuba abaphandi bafake i-pipette ngomlomo njengangaphambili. Loo ndlela yakudala yemibhobho ayikhuthazwa kuba inamandla okwenza iziphumo ezibi xa ulwelo lufunxwa emlonyeni.

Ibhola ye-pipette luhlobo lwe-pipette ngokuchaneka okubi kakhulu. Ngokuqhelekileyo idibaniswe ne-pipette yeglasi ukuhambisa inani eliguquguqukayo lolwelo.

Iimpompo zePipette nazo zifanelekile kwiipayipi zeglasi, ezinokudlulisela imiqulu yolwelo echanekileyo ngakumbi. Iimpompo zePipette zifanelekile ngokubanzi ukukhupha umthamo ofanayo wolwelo ngokuphindaphindiweyo.

Iipayipi ezincedisayo ziyi-pipettes eziqhelekileyo. Iqukethe iindawo ezininzi eziphambili: umlomo apho i-pipette ifakwe khona kwaye i-membrane yokucoca ifakwe, ekhusela ngaphakathi kwe-pipette yomncedisi ekungcoleni kwamanzi.

Amaqhosha amabini angabonwa kwi-handle ye-pipette yomncedisi. Xa iqhosha eliphezulu licinezelwe, i-liquid is aspirated, kwaye xa iqhosha elisezantsi licinezelwe, ulwelo luyakhutshwa.

Uninzi lweepipettes ezincedisayo nazo zineqhosha lokulawula izinga lokukhutshwa kwamanzi. Umzekelo, inokumiselwa ukukhulula ulwelo phantsi koxinzelelo, okanye isetelwe ukukhutshwa komxhuzulane ngaphandle kwamandla angaphandle.

Nangona ezinye iipayipi ezincedisayo ziza kunye nentambo yamandla, ininzi ibhetri inamandla.

Ezinye iipayipi ezincedisayo ziza kunye nesigxina esihambelana nendawo yokubamba, evumela ukuba i-pipette yomncedisi ibekwe kwicala layo xa ingasetyenziswanga ngaphandle kokususa i-pipette.

Njengoko kukhankanyiwe ngaphambili, i-pipette efanayo ingasebenzisa ubukhulu obuhlukeneyo beepipettes ngokuxhomekeke kumthamo oza kufakwa kwipayipi, ukusuka kwi-0.1 milliliters ukuya kwishumi leemililitha.

Ukusebenza okusisiseko kweepipettes

Okokuqala, khetha i-pipette yobungakanani obufanelekileyo ngokusekelwe kumthamo wolwelo ofuna ukuwahambisa. Emva koko vula ipakethe ukusuka phezulu, uthinte kuphela inxalenye engaphezulu kwophawu lophawu, uyifake kwincam yepipette, kwaye ususe iphakheji eseleyo.

Emva koko, bamba i-pipette ngesandla esinye kwaye uvule isiciko sesitya esiqulethe i-liquid ofuna ukuyinqwenela. Ukugcina i-pipette ngokuthe tye, cofa ngobunono iqhosha eliphezulu ukuze unqwenele isampulu yakho ngokucothayo.

Sebenzisa umgca ophumeleleyo eludongeni lwepipette ukulinganisa umthamo wolwelo ofuna ukuwahambisa. Qaphela ukuba umthamo kufuneka ufundwe phantsi kwe-meniscus, kungekhona phezulu.

Emva koko ukhulule ngononophelo ulwelo kwisikhongozeli osikhethileyo, ulumke ungavumeli incam yepipette ichukumise nawuphi na umphezulu ongeyontsholongwane.

Sebenzisa isilumkiso kunye namandla athambileyo xa ugxotha ulwelo, ngakumbi xa usebenzisa i-pipettes umthamo omncinci, ukuphepha ukungcolisa i-pipette yomncedisi kunye nesampuli, okanye ukonakalisa i-pipette yomncedisi. Ukungaphathwa kakuhle xa usebenzisa i-pipette yomncedisi unokucaphukisa abanye abantu abanamava ngakumbi kwilebhu, abanokufuneka bathathe i-pipette ngaphandle kokulungiswa. Xa ukupompa ubuninzi be-liquid okanye ukukhupha ulwelo, isantya sokudlulisa ulwelo sinokunyuswa ngokucinezela iqhosha kanzima.

Ekugqibeleni, khumbula ukulahla ngokufanelekileyo i-straw emva kokudlulisa ulwelo.

Isicelo

Ngoku ukuba uyayazi indlela yokusebenzisa i-pipette, makhe sijonge ngakumbi kwezinye izicelo eziqhelekileyo zaselabhoratri.

Isinyathelo esibalulekileyo xa ukulima kunye neeseli ze-plating kukusabalalisa okufanayo kweeseli kwisisombululo sokugqibela. Ukumiswa kweeseli kungaxutywa ngobumnene nangempumelelo usebenzisa i-pipette, edibanisa ngaxeshanye izisombululo zeekhemikhali kunye ne-reagents.

Emva kokuhlukaniswa okanye ukuqhutyelwa kweeseli zovavanyo, ii-pipettes zingasetyenziselwa ukudlulisa ii-clones zeeseli ezipheleleyo zokwandiswa okanye uhlalutyo lovavanyo olulandelayo.