Iiglasi ezitsha okanye ezisetyenzisiweyo kufuneka zifakwe emanzini kuqala ukuze zithambe kwaye zinyibilikise izixhobo.I-glassware entsha kufuneka ihlanjwe ngokulula ngamanzi ompompo ngaphambi kokusetyenziswa, kwaye emva koko ifakwe ubusuku bonke nge-5% ye-hydrochloric acid;I-glassware esetyenzisiweyo isoloko idityaniswe nenani elikhulu leprotheyini kunye negrisi, yomile emva kokuba akukho lula ukuyihlambulula, ngoko kufuneka ifakwe ngokukhawuleza emanzini acocekileyo ukuze ixutywe.
1. Imiba efuna ukuqwalaselwa:
Emva kokucoca kunye ne-disinfection ngaphambi kokusetyenziswa, nokuba i-petri dish ihlambulukile okanye ayinayo impembelelo enkulu emsebenzini, inokuchaphazela i-ph ye-culture medium, ukuba kukho iikhemikhali ezithile, ziya kuthintela ukukhula kweebhaktheriya.
Izitya zepetri ezisanda kuthengwa kufuneka zihlanjululwe ngamanzi ashushu kuqala, kwaye emva koko zifakwe kwisisombululo se-hydrochloric acid kunye neqhekeza elikhulu le-1% okanye i-2% kwiiyure ezininzi ukususa izinto ze-alkaline zamahhala, kwaye zihlanjululwe ngamanzi adibeneyo kabini.
Ukuba ufuna ukuvelisa iibhaktheriya, ke sebenzisa umphunga woxinzelelo oluphezulu (jikelele 6.8 * 10 5 Pa umphunga woxinzelelo oluphezulu), inzala kwi-120 ℃ ye-30min, yomile kwiqondo lobushushu begumbi, okanye ubushushu obomileyo, kukubeka isitya se-petri kwi-oveni. , ulawulo lobushushu malunga ne-120 ℃ phantsi kwemeko ye-2h, unokubulala izinyo lebhaktheriya.
Izitya ze-petri ezizintsholongwane zingasetyenziselwa ukugonywa kunye nenkcubeko.
2. Sebenzisa indlela:
Beka ibhotile ye-reagent ukuze isetyenziswe kwindawo efanelekileyo kwindawo yokusebenza, kwaye ukhulule i-cap yebhotile ye-reagent ukuze isetyenziswe.
Beka izitya zepetri embindini wendawo yakho yokusebenza;
Susa i-cap yebhotile ye-reagent kunye ne-siphon i-reagent kwibhotile ye-reagent nge-pipette.
Beka isiciko sesitya sepetri ngasemva kwayo;
Ngenisa ngobunono i-culture medium ngqo kwisiseko secala elinye lesitya;
Beka isiciko kwisitya se-petri;
Beka isitya kwicala layo, uqaphele ukuba ungavumeli i-medium ingene kwindawo encinci phakathi kwesivalo kunye nezantsi;
Susa umququ osetyenzisiweyo.
Ixesha lokuposa: Apr-26-2022